Technical assistant 3 2 checking.
Waters hplc system cleaning procedure.
To clearly lay down the procedure for cleaning.
Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.
When working with any lc system setting it up and storing it is very important.
For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
Responsibility quality control officer executive.
Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307.
2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.
Reverse osmosis to remove most contaminants b.
Cleaning decontamination columns general guidelines flow cells general.
To clean the system.
Ion exchange to remove any remaining ions.
Use only hplc grade solvents and water filtered through 0 2.
Use solvent inlet filters to protect the system from incoming particles.
To clean the system.
Water flush to clear customer s mobile phases from the system.
Remove all solvent filters.
Label bottles correctly with bottle content and filling date expiry date.
10 nh4oh in water this removes peg and other.
Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.
The purification process should include all of the following steps.
The water flushes are only needed to remove the previous solvent from the system.
This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules.
Water flush to clean acid from the system.
Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa.
Maintaining your hplc uhplc uplc systems.
Disconnect the column and attach a union and flow directly to waste.
It is necessary to restore the system flow path to a near new level of cleanliness note.
Place a b c and d seal wash and needle wash lines into a clean.
No injections are needed with that.
Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.
Controlling contamination in lc ms and hplc ms systems 715001307.
